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Fig. 4 | Respiratory Research

Fig. 4

From: Intrapleural dual blockade of IL-6 and PD-L1 reprograms CAF dynamics and the tumor microenvironment in lung cancer-associated malignant pleural effusion

Fig. 4

IL-6 increased CAF infiltration in the MPE mouse model. Comparison of IL-6 levels in mice MPE A with serum and B between groups. C The percentage of the overall CAFs (CD45−CD90+) in mice tumor nodules were quantified by flow cytometry. D Representative images of IHC staining of α-SMA, PDGFRα, podoplanin, and FAP in MPE mice tumor nodules. Scale bar = 40 µm. E Western blot analysis of p-STAT3, STAT3, and α-SMA expression in CAFs treated with IL-6, MPE, MPE and tocilizumab. β-tubulin was used as the equal loading control. All experiments were performed with ≥ 3 biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not statistically significant. BSE: blood serum; MPE: malignant pleural effusion; FAP, fibroblast activation protein-α; α-SMA, α-smooth muscle actin; PDGFRα, platelet derived growth factor receptor α; CAFp1, CAFs isolated from patient 1; CAFp2, CAFs isolated from patient 2; TCZ, tocilizumab

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Respiratory Research

ISSN: 1465-993X

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