Fig. 6
From: SERPINB10 promotes macrophage M2 polarization and airway inflammation in asthma
SERPINB10 suppresses IL-4Rα degradation and upregulates IL-4R signaling in macrophages. A Representative image of western blotting for phosphorylated Stat6 (p-Stat6) at different time points after IL-4 stimulation in BMDMs. B Densitometry assay was performed using Image J to quantify the protein levels of p-Stat6. C Representative image of western blotting for phosphorylated Akt (p-Akt) at different time points after IL-4 stimulation in BMDMs. D Densitometry assay was performed using Image J to quantify the protein levels of p-Akt. E Representative image of western blotting for IL-4Rα in THP-1 cells after transfection with negative control or SERPINB10 siRNA and stimulated with or without IL-4. F Densitometry assay was performed using Image J to measure the protein levels of IL-4Rα. G Representative image of western blotting for IL-4Rα in THP-1 cells transfected with negative control or SERPINB10 siRNA and treated with cycloheximide for different times as indicated. H Densitometry assay was performed using Image J to measure the protein levels of IL-4Rα. Data are mean ± SD. *P < 0.05, **P < 0.01, and ****P < 0.0001