Fig. 2

IPAH monocyte differentially expressed genes (DEG) reveal a CD14^low/STAT1^high signature. Differential expression (DE) analysis was performed on Control vs. IPAH classical, intermediate, and non-classical. (A-C) Volcano plots of DE genes, comparing fold change (FC) in genes up and down regulated in Control vs. IPAH. Orange dots represent upregulated genes in IPAH monocytes (adjusted p < 0.01 and FC ≥ 2), blue dots represent downregulated genes in IPAH monocytes (p < 0.01 and FC ≤ 0.5). (D) Venn diagram showing number of DE genes in each monocyte subpopulation, identifying common DE genes for all three subpopulations in IPAH compared to Control. (E) Heat map of individual DE genes and molecular pathway each gene is implicated in, for the DE genes common to all three monocytes subpopulations. (F) Heat map of individual DE genes and molecular pathway each gene is implicated in, for the 26 DE genes common to the classical and intermediate monocytes subpopulations. n = 6 IPAH patients or Controls. In (E and F), C: classical, I, intermediate, and NC, non-classical monocytes