Table 1 Antibodies and staining materials used in the present study
From: Integrin α8 is a useful cell surface marker of alveolar lipofibroblasts
Target protein | Supplier | Code | Application | Dilution |
|---|---|---|---|---|
ITGA 8 (biotin-conjugated) | R&D | BAF4076 | FACS/IHC | 1:100 |
Ly6a (SCA-1) (PE/Cy7) | eBioscience | 25-5981-82 | FACS | 1:100 |
CD140a (PDGFRA) (BV421) | BioLegend | 135923 | FACS | 1:50 |
CD326 (EpCAM) (APC or PE/Cy7) | eBioscience | 17-5791-82 | FACS | 1:100 |
CD31 (APC or PE/Cy7) | BioLegend | 102410 | FACS | 1:100 |
CD45 (APC or PE/Cy7) | BioLegend | 103112 | FACS | 1:100 |
CD146 (APC) | BioLegend | 134712 | FACS | 1:100 |
LYVE-1 (APC) | R&D | FAB2125A | FACS | 1:100 |
CD90.2 (THY-1.2) (biotin-conjugated) | BioLegend | 140314 | FACS | 1:100 |
PE Streptavidin | BioLegend | 405203 | FACS | 1:100 |
Ly6a (Sca-1) | Miltenyi Biotec | 130-123-848 | IHC | 1:100 |
Prosurfactant prontein C | Merck | AB3786 | IHC | 1:500 |
DAPI | Nakalai | 19178-91 | FACS/IHC | 1:500 |
SYTOX Red | Thermo Fisher | S34859 | FACS | 1:1000 |